6/28/2023 0 Comments Author of dark emu![]() ![]() ![]() Result: We screened the key targets of EMO against RA using network pharmacology methods, including HMGB1, STAT1, EGR1, NR3C1, EGFR, MAPK14, CASP3, CASP1, IL4, IL13, IKBKB and FN1, and their reliability was verified using ROC curve. ![]() Finally, RNA-seq analyses were conducted on synovial fibroblasts from EMO-treated. To further investigate the anti-RA effect of EMO on MH7A cells, the expression of IL-6 and IL-1β were monitored. Further, single cell RNA sequencing data from GEO database of RA patients (GSE159117) were downloaded and analysed. Methods: A gene expression profile from GSE55457 available from the Gene Expression Omnibus (GEO) database was used to identify the targets of EMO action. In order to explore the underlying mechanism of action of EMO against rheumatoid arthritis (RA), a network pharmacology approach was employed. Introduction: Emodin (EMO), a natural derivative of the anthraquinone family mainly extracted from rhubarb (Rheum palmatum), has previously been demonstrated to possess superior anti-inflammatory properties from a single target or pathway. 2The First Clinical College, Hubei University of Chinese Medicine, Wuhan, China.1Department of Integrated Traditional Chinese and Western Medicine, The First Affiliate Hospital of Chongqing Medical University, Chongqing, China.Pusheng Hui 1 †, Sicong Zhou 1 †, Chunhao Cao 1 †, Wenting Zhao 2, Li Zeng 1* and Xiaofeng Rong 1* ![]()
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